Josie Given, Rina Pigozzo, Jim Fairles
Jim Fairles - from AHL LabNote 36
One of the most important aspects of diagnostic work is the actual sampling and handling of the samples prior to being received at the diagnostic laboratory.
The best sample for microbiology testing is usually the substrate itself – feces, tissue, or fluid in leak-proof containers (liquids, feces) or leak-proof Whirl-Pak bags (tissue, feces) (Ziplock bags are discouraged!).
Bacteriology and Mycoplasmology - AHL prefers a regular gel-based bacteriology swab.
Virology - If swabs are needed, AHL prefers virus transport medium swabs for virology PCR tests. For some testing (PEDV), a fecal swab in VTM is preferred rather than feces. Regular gel-based bacteriology swabs are unsuitable for PCR testing. Dry swabs moistened with sterile saline in a sterile container can be used, but VTM is preferred.
The VTM swabs pictured above are also available from the AHL for $2.50 – please email ahlinfo@uoguelph.ca to order.