TEST NAME METHOD	CODE	SPECIES
Avian adenovirus - PCR	fadvrrt	Avian
Avian metapneumovirus type C (AMPV type C) - PCR	ampvpcr	Avian
Bacillus anthracis (anthrax) - real-time PCR	anthpcr	Bovine
Bacterial culture, aerobic and anaerobic, food animal	ancultf	Av, Bov, Cap, Ov, Porc, Other
Bacterial culture, honey bee, American foul brood (P. larvae)	cultbeo	Bees
Antimicrobial susceptibility, honey bee, Paenibacillus larvae	afbo	Bees
Bacterial culture, fecal, equine	cultnf1	Equine
Bacterial culture, fecal, Campylobacter, add-on test	campadd	Av, Bov, Cap, Eq, Other, Ov, Porc
Bovine abortion panel - PCR (BoHV-1/IBR, Leptospira, Neospora)	boabopc	Bovine
Bovine enteric panel - BCoV/RotaV A/B PCR; sucrose wet mount, bacterial culture	bentpnl	Bovine
Bovine respiratory virus panel - PCR (BoHV-1/IBR, BPIV-3, BRSV)	brvp3	Bovine
Bromethalin (desmethylbromethalin) - LC-MS/MS	dmb	Av, Bov, Can, Cap, Eq, Fel, Other, Ov, Porc
Haemoplasma, non-feline, non-canine - PCR                                            Mycoplasma haemolamae, M. ovis, M. suis, M. wenyonii	hapcr2	Bovine, Other, Porcine
Leptospira spp. - PCR	leptpcr	Bov, Can, Cap, Eq, Fel, Other, Ov, Porc
Mycoplasma hyopneumoniae - gene sequencing typing	p146seq	Other, Porcine
Myxobolus cerebralis (whirling disease pathogen) - PCR	wdpcr	Fish
Rotavirus A&B/coronavirus, bovine/equine - PCR	rocopcr	Bovine, Equine
Rotavirus, group A, B, C – PCR	rotapcr	Canine, Other, Porcine
Scrapie resistance PrP genotyping, codons 136, 137, 154, 171, 176  - sequencing	prp5	Ovine

 

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Selected zoonotic pathogens and diseases from Ontario identified at the AHL, 2017  

Beverly McEwen, Durda Slavic, Davor Ojkic, Hugh Cai, Kristiina Ruotsalo, Josepha DeLay, Margaret Stalker, Murray Hazlett, Andrew Brooks, Janet Shapiro, Maria Spinato, Marina Brash, Emily Martin

Many new, emerging, and re-emerging diseases of people are caused by pathogens originating from animals, or are shared between people and animals. The AHL plays an important role in public health by identifying zoonotic pathogens in > 1,000 cases annually (Tables 1 and 2). The number and percentage of cases identified as positive for leptospirosis increased in 2017 in all species tested. These are numerator data reliant upon submission biases to the diagnostic laboratory and cannot be regarded as population prevalence estimates. Monitoring programs are not included.   AHL

Table 1. Number of cases with selected zoonotic pathogens isolated and/or identified at the AHL, 2017.

Agent	Bovine	Swine	Equine	Ovine	Caprine	Chicken	Turkey	Canine	Feline	Other	2017	2016	2015	2014	2013	2012
Ascarids (T. canis, T. cati, T. leonina, Baylisascaris sp.)	0	12	10	0	0	23	2	16	5	9	77	41	17	40	36	35
Blastomyces dermatitidis	0	0	0	0	0	0	0	13	1	2	16	6	21	22	17	10
Bordetella bronchiseptica	3	24	3	0	0	0	0	4	4	12	50	32	37	28	24	33
Borrelia burgdorferi (Lyme disease), serology	0	0	20	0	0	0	0	9	0	0	29	22	8	12	11	3
Brucella sp. (non-abortus)	0	0	0	1	0	0	0	0	0	0	1	3	0	1	0	0
Campylobacter coli/ jejuni/ fetus subsp. fetus	3	0	0	5	5	20	3	3	0	5	44	47	16	17	6	17
Chlamydia sp.	1	0	0	5	11	0	0	0	0	1	18	13	24	15	25	33
Clostridium difficile	1	8	4	0	0	0	0	0	0	1	14	5	10	11	11	19
Coxiella burnetii (Q fever)	9	0	0	39	54	0	0	0	0	0	102	37	44	55	28	36
Cryptococcus sp.	0	0	1	0	0	0	0	0	0	0	1	1	1	3	2	1
Cryptosporidium sp.	172	0	1	1	5	0	0	0	0	1	180	243	247	186	206	141
Eastern equine encephalitis virus	0	0	2	0	0	0	0	0	0	0	2	0	6	25	1	0
Giardia sp.	5	0	0	0	0	0	0	21	0	0	26	26	30	50	48	26
Listeria monocytogenes	19	0	0	4	8	0	0	0	0	0	31	24	12	23	15	18
Methicillin-resistant Staphylococcus aureus (MRSA)	2	0	9	0	0	0	0	0	0	0	11	12	28	17	8	24
Methicillin-resistant S. pseudintermedius (MRSP)	0	0	0	0	0	0	0	72	4	0	76	62	88	45	141	114
Rabies virus	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0
Salmonella enterica	64	67	8	8	3	27	35	24	2	28	266	290	332	221	308	281
Streptococcus suis	10	102	2	2	1	1	1	0	0	1	120	181	167	105	126	144
Streptococcus equisimilis	4	28	14	1	0	0	0	8	1	3	59	57	48	4	34	45
Streptococcus zooepidemicus	0	2	121	2	0	0	0	2	2	4	133	154	138	93	112	4
Toxoplasma sp.	0	0	0	6	4	0	0	0	2	0	12	7	11	18	11	8
Verotoxigenic E.coli (VTEC)	5	0	0	0	1	0	0	0	0	0	6	12	8	7	18
West Nile virus	0	0	15	0	0	0	0	0	0	43	58	18	19	6	44	36
Yersinia enterocolitica	2	6	0	0	0	0	5	0	0	0	13	4	2	6	4	2
Total	300	249	210	74	92	71	46	172	21	110	1,345	1,299	1,314	1,010	1,236	1,030

 

Table 2. Leptospira spp. seropositive, IHC-positive, or PCR-positive cases identified at the AHL, 2017.

Leptospira spp. serovar	Bovine	Swine	Equine	Canine	Other
L. autumnalis	27	7	18	86	2
L. bratislava	72	7	20	74	3
L .canicola	39	7	8	50	0
L. grippotyphosa	16	6	4	71	1
L. hardjo	50	7	3	31	2
L. icterohaemorrhagiae	55	6	15	82	1
L. pomona	64	7	11	71	5
IHC, or urine PCR-positive	1	1	0	10	0
Positive/tested cases	91/178	8/27	26/35	153/230	5
% positive, 2017/2016	51/27%	30/9%	74/52%	67/38%

 

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AHL Newsletter, March, 2018 - Volume 22, Number 1

       Editor:  Grant Maxie, DVM, PhD, Diplomate ACVP

       Editorial Assistants:  Helen Oliver, April Nejedly

The AHL Newsletter is published quarterly (March, June, September, December) by the Animal Health Laboratory, Laboratory Services Division, University of Guelph.

Its mission is to inform AHL clients and partners about AHL current activities, and laboratory-based animal disease events and disease trends.  All material is copyright 2018.  Ideas and opinions expressed herein do not necessarily reflect the opinions of the University or the Editor.

Articles may be reprinted with the permission of the editor and with appropriate credit given to the AHL Newsletter.

Mailing address & contact information:

Animal Health Laboratory

Laboratory Services Division, University of Guelph

Box 3612, Guelph, Ontario, Canada  N1H 6R8

Phone: (519) 824-4120 ext. 54538; fax: (519) 821-8072

To receive an electronic copy of this Newsletter, please send your email address to us at holiver@uoguelph.ca

ISSN 1481-7179

Canada Post Publications number - 40064673

Contributors to this issue

- from the Animal Health Laboratory:

Melanie Barham, DVM, PMP

Marina Brash, DVM, DVSc, Diplomate ACVP

Andrew Brooks, DVM, PhD, Diplomate ACVP

Emily Brouwer, HBSc, DVM, Diplomate ACVP

Hugh Cai, DVM, MSc, DVSc

Michael Deane, BA

Josepha DeLay, DVM, DVSc, Diplomate ACVP

Jim Fairles, DVM, MBA

Murray Hazlett, DVM, DVSc, Diplomate ACVP

Emily Martin, DVM, MSc, Diplomate ACPV

Beverly McEwen, DVM, PhD, Diplomate ACVP

Davor Ojkic, DVM, PhD

Felipe Reggeti, DVM, PhD, Diplomate ACVP

Kristiina Ruotsalo, DVM, DVSc, Diplomate ACVP

Janet Shapiro, DVM, DipEqSurg, DipPath

Durda Slavic, DVM, PhD

Maria Spinato, DVM, DVSc, Diplomate ACVP

Margaret Stalker, DVM, PhD, Diplomate ACVP

Qiumei You, DVM, MSc

Other contributors:

Leonardo Susta, DVM PhD, DACVP, Pathobiology;, OVC.

Al Dam, BSc; Andrew Vince, DVM, DVSc, Diplomate ACVP; OMAFRA, Guelph, ON .

Our continued thanks to all of the non-author AHL clerical, technical, and professional staff who contribute to the generation of results reported in the AHL Newsletter.