Amr El-Shalofy

DVM, MS, PhD (Switzerland)

aelshalo@uoguelph.ca
Office: Stewart Bldg.2502
226-979-0089

Profile

Dr. Amr El-Shalofy is a dedicated equine reproduction veterinarian and clinical researcher, who grew up in Damietta in northern Egypt. After graduating with honors from Cairo University's Faculty of Veterinary Medicine, he pursued his passion for horses by earning a doctorate in equine endometritis from the University of Zurich in Switzerland.
His clinical journey began with an internship at the Paul Schockemöhle Stud in Germany, where he specialized in equine reproduction. Over the past nine years, he has built an extensive career, practicing and teaching at leading universities and stud farms across Switzerland, Germany, Austria, and Belgium. His professional interests center on assisted reproductive technologies and infertility management in mares.
Outside of his veterinary work, Amr enjoys painting, biking, and spending quality time with his wife, Mona, and their son, Anas.

Research Interests

Dr. El-Shalofy’s research integrates reproductive physiology, biotechnology, and clinical theriogenology, with a focus on improving fertility management and reproductive efficiency in equines and livestock. His work bridges basic science with applied veterinary practice, emphasizing molecular biology, cryopreservation, and assisted reproductive technologies.

Key Research Areas

Equine Reproductive Health

• Investigating genetic markers associated with susceptibility to persistent breeding-induced endometritis in mares through transcriptomic analyses.
• Optimizing semen preservation techniques and exploring shipping protocols for cryopreserved horse semen.

Reproductive Biotechnology and Cryopreservation

• Advancing cryopreservation of oocytes in buffaloes and camels by exploring novel vitrification techniques and cryoprotectants.
• Enhancing in-vitro fertilization (IVF) outcomes and embryo development through improved oocyte preservation strategies.

Testicular and Uterine Hemodynamics

• Evaluating hormonal and pharmacological interventions (e.g., L-arginine, melatonin, oxytocin) to boost reproductive performance under environmental and seasonal stressors.
• Studying testicular blood flow, echotexture, and endocrine responses in small ruminants.

Fertility Management in Livestock

• Applying reproductive synchronization protocols (e.g., Ovsynch) to improve fertility in buffalo cows.
• Investigating the influence of nutritional and environmental factors on reproductive health and semen quality in bulls, rams, and goats.
  

Teaching

VETM*3460 – Theriogenology
VETM*4530 Health Management III

Selected Publications

1. Khan, Y., El-Shalofy, A., Kaps, M., Gautier, C., & Aurich, C. (2024). In mares resistant to endometrial infection, periovulatory treatment with ecbolic drugs does not influence uterine clearance or luteal development. Animal Reproduction Science, 267, 107548.
2. El-Shalofy, A.S., Gautier, C., Khan, Y., Aurich, J., Aurich, C., (2023). Shipping duration and temperature influence the characteristics of cryopreserved horse semen stored in different shipping devices for up to 14 days. Animal Reproduction Science.
3. El-Sherbiny, H. R., El-Shalofy, A. S., & Samir, H. (2023). Association between body condition score, testicular hemodynamics and echogenicity, nitric oxide levels, and total antioxidant capacity in rams. Irish Veterinary Journal, 76(1), 1-7.
4. Yaqout, K. A., Bard, M. R., El-Wishy, A. B. A., Moawad, A. R., & El-Shalofy, A. S. (2023). Influences of glycine supplementation during vitrification on the developmental potential of vitrified/warmed immature dromedary camel oocytes. Reproduction in Domestic Animals, 58, 614– 621.
5. Samir, H., El-Sherbiny, H. R., & El-Shalofy, A. S. (2023). Seasonal Alterations in Testicular Hemodynamics and Echotexture in Relation to Semen Quality in Buffalo Bulls. Andrologia, 2023.
6. El-Shalofy, A. S., Samir, H., & El-Sherbiny, H. R. (2023). Intramuscular administration of l-arginine boosts testicular hemodynamics, plasma concentrations of testosterone and nitric oxide in heat-stressed rams. Theriogenology, 197, 127-132.
7. Samir, H., El-Shalofy, A. S., & El-Sherbiny, H. (2023). Effects of a single dose of long-acting FSH on testicular blood flow, testicular echotexture, and circulating testosterone, estradiol, and nitric oxide in rams during the non-breeding season. Domestic Animal Endocrinology, 82, 106765.
8. Hedia, M. G., & El-Shalofy, A. S. (2022). Oxytocin improves testicular blood flow without enhancing the steroidogenic activity in Baladi goats. Asian Pacific Journal of Reproduction, 11(5), 223.
9. El‐Sherbiny, H. R., Samir, H., El‐Shalofy, A. S., & Abdelnaby, E. A. (2022). Exogenous L‐arginine administration improves uterine vascular perfusion, uteroplacental thickness, steroid concentrations and nitric oxide levels in pregnant buffaloes under subtropical conditions. Reproduction in Domestic Animals.
10. Yaqout, K. A., Monir, A., Badr, M. R., Abou Bakr, A., Moawad, A. R., & El-Shalofy, A. S. (2022). Impact of presence or absence of trehalose during vitrification on viability and development of vitrified/warmed immature dromedary camel oocytes. Open Journal of Animal Sciences, 12(3), 347-359.
11. El-Shalofy, A., Wagener, K., Weber, K., Blanco, M., Bauersachs, S., & Bollwein, H. (2022). Identification of genes associated with susceptibility to persistent breeding-induced endometritis by RNA-sequencing of uterine cytobrush samples. Reproductive biology, 22(2), 100577.
12. El-Shalofy, A. S., Shahat, A. M., & Hedia, M. G. (2022). Effects of melatonin administration on testicular hemodynamics, echotexture, steroids production, and semen parameters during the non-breeding season in Ossimi rams. Theriogenology, 184, 34-40.
13. El-Sherbiny, H. R., El-Shalofy, A. S., & Samir, H. Exogenous L-carnitine administration ameliorates the adverse effects of heat stress on testicular hemodynamics, echotexture, and total antioxidant capacity in rams. Frontiers in Veterinary Science, 349.
14. Hedia, M., & El‐Shalofy, A. (2021). Ageing affects plasma steroid concentrations and testicular volume, echotexture, and hemodynamics in rams. Andrologia, e14309.
15. El-Sherbiny, H., Shahat, A., Hedia, M., & El-Shalofy, A. (2022). Effect of sexual maturation on testicular morphometry and echotexture and their association with intratesticular blood flow in ossimi rams. Indian Journal of Small Ruminants, 28(1), 85-90.
16. El‐Shalofy, A.S., Hedia, M., & Kastelic, J. (2021). Melatonin improves testicular hemodynamics, echotexture, and testosterone production in Ossimi rams during the breeding season. Reproduction in Domestic Animals.
17. El‐Shalofy, A. S., & Hedia, M. G. (2021). Exogenous oxytocin administration improves the testicular blood flow in rams. Andrologia, 53(10), e14193.
18. El‐Shalofy, A. S., & Hedia, M. G. (2021). Effects of buserelin administration on testicular blood flow and plasma concentrations of testosterone and estradiol-17β in rams. Domestic Animal Endocrinology, 106646.
19. El‐Shalofy, A. S., (2020). Transcriptomics of the endometrium in mares susceptible and resistant to persistent breeding-induced endometritis using RNA-Sequencing (Doctoral dissertation, University of Zurich).
20. El-Shalofy, A. S., Derbala, M. K., Asfour, H. A., Eissa, H. M., & Aly, A. B. (2021). Infectious endometritis in Arabian mares: an updated clinical investigation of uterine microbial isolates, antimicrobial sensitivities, and fertility in Egypt. The Thai Journal of Veterinary Medicine, 51(1), 177-184.
21. El-Shalofy, A. S., Ismail, S. T., Badawy, A. A., Darwish, G. M., Badr, M. R., & Moawad, A. R. (2020). Effect of Disaccharide Inclusion in Vitrification and Warming Solutions on Developmental Competence of Vitrified/Warmed Germinal Vesicle Stage Buffalo Oocytes. Cryoletters, 41(6), 351-357.
22. El-Shalofy, A. S., Moawad, A. R., Darwish, G. M., Ismail, S. T., Badawy, A. B., & Badr, M. R. (2017). Effect of different vitrification solutions and cryodevices on viability and subsequent development of buffalo oocytes vitrified at the germinal vesicle (GV) stage. Cryobiology, 74, 86-92.