Bottle-feeding a baby bee, aka inoculating a newly emerged bee, is the most critical method of honey bee’s immunity study against nosema, a microsporidian parasite in the midgut. The newly emerged bee will be bottle-fed with mixed sugar syrup and nosema spores to make sure nosema spores will infect them at the same level. This experiment was conducted with more than 4,760 bees in the summer of 2022. We fed any single of them one at a time with the help of the research team at the Honey Bee Research Centre.