DNA Sequencing
Sample submission considerations. [1]
The facility provides a number of standard sequencing primers [2] for common plasmid vectors.
Custom primers should be provided at a concentration of 5 uM (=5 pmol/uL).
Request Forms:
Individual Sample Submissions
Sanger Sequencing Request [3] - Use this form to submit DNA templates (ie. PCR amplicons, Plasmids, etc.) for sequencing
DIY Sequencing Request [4] - Use this for to submit completed flourescent Sanger products (ie. BigDye reactions) for sequencing
For full and half-plate submissions please contact the facility
Equipment Used: Applied Biosystems 3730 DNA analyzer [5]