Quality Assurance
Accurate and fast
Sequencing
Competitive pricing for cutting-edge instruments
Analysis
Publication-ready figures
Plasmid & PCR Amplicon Sequencing
We have phased out Sanger Sequencing in favor of long-read sequencing on the Oxford Nanopore PromethION 24. The facility provides sequencing of DNA fragments up to 25 kilobases in length or more, including whole plasmids and PCR amplicons.
- Sequenced at the University of Guelph: no tax or shipping fees for internal clients
- Plasmid analysis run on Tuesdays and Thursdays with next-day turnaround
- Pricing includes bioinformatic report, plasmid annotation, insert variant calling, and multiplasmid/contamination detection.
- See our detailed sample report
| SERVICE | INTERNAL | ACADEMIA | COMMERCIAL |
|---|---|---|---|
| PromethION | |||
| Plasmid (1 Kb reads) Sequencing | $19.50 ea; $17 ea for 48+ samples batched and submitted at once | $25.00 | $25.00 |
| Amplicon (1K reads) Sequencing | $19.50 | $25.00 | $25.00 |
| Amplicon (4K reads) sequencing | $30.00 | Contact Facility | Contact Facility |
Contact the facility to discuss project design and sample requirements.
Sequencing Request Form - Use this form to submit DNA templates (e.g. PCR amplicons, Plasmids) for sequencing. Recommended at least 100 ng DNA. Recommended concentration 10 ng/µL in 10 µL in molecular grade water or TE buffer. Concentration should be estimated by fluorometric methods such as Qubit/PicoGreen.
- Fill out all three tabs of the request form (first two tabs are mandatory)
- Signed request form emailed to genomics@uoguelph.ca
- Drop the samples off at room SSC 1401 with facility staff or in the Sample Drop-Off Freezer
PromethION Sequencing
The PromethION 24 is a high-throughput nanopore sequencer capable of natively sequencing both RNA and DNA with rapid turn-around times. The PromethION can load up to 24 flow cells, each capable of multiplexing 96 samples or more. It is also possible to re-use flow-cells, decreasing cost and waste. The PromethION 24 is suitable for long-read applications, including genome assembly, native methylation seq., direct RNA transcript sequencing (including alternate splicing), and metagenomics.
| SERVICE | INTERNAL | EXTERNAL |
|---|---|---|
| PromethION | ||
| Plasmid (1 Kb reads) Sequencing | $19.50 ea; $17 ea for 48+ samples batched and submitted at once | $25.00 |
| Amplicon (1K reads) Sequencing | $19.50 | $25.00 |
| Amplicon (4K reads) sequencing | $30.00 | |
| Full length 16S or other community metabarcoding (50K reads), includes taxonomic classification | $45 (+$10/sample for PCR if not already amplified, +$10/sample for QC/normalization) | |
| Shotgun Metagenomics (1 Gbp) | $125 | |
| Bacterial Whole Genome Sequencing (400 Mbp) | $100 ea; $75 ea for 48+ samples batched and submitted at once | |
| Whole flow cell for DIY libraries (80–100 Gbps+) | $1,200 | |
| Optional PromethION Sample Processing | ||
| Purification and Quantification + Sample Normalization | $10 | |
| PCR Amplification of low-input sample | $10 | |
Contact the facility to discuss project design and sample requirements.
Illumina Sequencing Fees
The Illumina NextSeq 1000 is an NGS sequencing system designed to miniaturize sample and reagent volumes and increase read output. The NextSeq 1000 is capable of many sequencing applications, including targeted metagenomics, whole genome sequencing, transcriptomics, immune repertoire profiling, sequencing of targeted panels, exomes and large panels, circulating tumor DNA, single-cell sequencing, and spatial profiling.
Contact the facility to discuss services and applications.
| SERVICE | INTERNAL | EXTERNAL |
|---|---|---|
| Library prep. & seq. | ||
| Amplicon library preparation and sequencing (250k reads/sample, 2x300 cycles) | $50 | Contact Facility |
| Amplicon library preparation and sequencing (50k reads/sample, 2x300 cycles) | $30 | Contact Facility |
| 16S Profiling (250k reads/sample, 2x300 cycles) | $50 | Contact Facility |
| Library Preparation | ||
| Illumina DNA library preparation | $130 | Contact Facility |
| RNA-Seq Library Prep (PolyA capture) | $115 | Contact Facility |
| RNA-Seq Library Prep (Ribodepletion) | $165 | Contact Facility |
| Library QC | $25 | Contact Facility |
| Sequencing | ||
| NextSeq cartridges | Contact Facility | |
| 300 cycle (1 x 150) P1 | Contact facility | Contact Facility |
| 600 cycle (2 x 300) P1 25M Reads | $1425 per 25M reads | Contact Facility |
| NovaSeq X | Contact Facility (est. installation June 2026) | |
Gene Expression Services
Request Forms:
Digital Droplet PCR Request
Real-Time PCR Registration
Real-Time PCR Request
QuantStudio 7 Pro Subscription
Equipment Used:
BioRad Qx200 Droplet Digital PCR (ddPCR) System
QuantStudio 7 Pro Real-Time PCR System
Digital Droplet PCR
Droplet Digital PCR technology is a digital PCR method utilizing a water-oil emulsion droplet system. Droplets are formed in a water-oil emulsion to form the partitions that separate the template DNA molecules.
| SERVICE | INTERNAL | EXTERNAL |
|---|---|---|
| One column (8 reactions) | $70 | $80 |
RT-PCR: QuantStudio 7 Pro
This system makes simple and easy to get high-quality real-time PCR results. This automated real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.
| SERVICE | INTERNAL |
|---|---|
| Full service | $3.00 per reaction (clients provide RNA samples and primers) |
| Self-service | $150 per year for registration and $7 per plate run |
| Consumables | |
| 96 Well qPCR Plate + Seal (pack of 10) | $70 |
| 384 Well qPCR Plate + Seal (pack of 10) | $90 |
| qPCR Seals (pack of 10) | $9 |
| SYBR Green (5 mL tube) | $130 |
DNA/RNA Preparation
Our facility offers DNA/RNA extraction, amplification, quantification, normalization, and clean-up services.
Contact the facility to discuss project design and sample requirements.
| SERVICE | INTERNAL |
|---|---|
| Extraction | $20 |
| PCR and Bead Clean up | $10 |
| Bead Clean up | $3 |
| Quantification and Normalization | $7 DNA; $13 RNA |
| Qubit | $3 standards per run + $1.50 per sample |
| Tapestation | See Tapestation section |
DNA/RNA Quantification
Qubit
Fluorometric concentration assays are generally more accurate than spectrophotometric/UV-Vis absorbance (ie. Nandrop) measurements because they are less affected by common contaminants (ie. Proteins, RNA, or DNA).
This method is recommended for NGS library assessments and for any application that requires higher accuracy in concentration estimates than can be achieved with Nanodrop.
Request Form:
Qubit Request
Equipment Used: Qubit Flex Fluorometer, Qubit® 2.0 Fluorometer
| SERVICE | INTERNAL |
|---|---|
| Qubit analysis | $1.50 per sample, plus $3 for standards |
Nanodrop
The NanoDrop® ND-8000 can measure the concentration and quality of RNA, DNA, protein or labelled probes for microarray analysis. It is designed for the use of 1 ul samples. Cuvettes are not required. NanoDrop® ND-8000 can measure 8 samples simultaneously.
Subscription Form
| SERVICE | INTERNAL |
|---|---|
| Nanodrop | $150 per year |
| Nanodrop & QuantStudio 7 Pro | $280 per year + $7 per plate run on QuantStudio |
Agilent TapeStation 4150
The TapeStation system allows for automated electrophoresis of most DNA and RNA samples. Typical applications include quality control (QC) of NGS libraries and RNA extractions (ie. RIN). This method excels in the determination of fragment length distribution of nucleic acid samples, but is not typically relied on for accurate quantification. The TapeStation can be used in place of the BioAnalyzer for most applications, but cannot currently be used for small/micro RNA analysis.
Service includes Standard and High Sensitivity assays for RNA, DNA >1kb, and DNA >5kb, as well as a Standard gDNA assay.
Request Form: AAC TapeStation Request Form
Equipment Used: Agilent TapeStation 4150
| Assay | Set-up | Per Sample |
|---|---|---|
| RNA | $10.50 | $5.50 |
| HS RNA | $12.00 | $6.25 |
| D1000 | $8.00 | $5.50 |
| HS D1000 | $11.00 | $7.75 |
| D5000 | $12.75 | $5.25 |
| HS D5000 | $17.25 | $7.75 |
| gDNA | $8.75 | $8.75 |
Set-up fee is a flat fee charged per submission in addition to the per sample charge.
Set-up fee covers DNA/RNA ladders, technician time, and instrument overhead. Contact facility for more information about ladders and when to use them.
About Us
The Genomics Facility is one of five highly specialized laboratories in the University of Guelph's Advanced Analysis Centre. The AAC is located in the multidisciplinary Summerlee Science Complex which provides a range of capabilities for research and advanced education at the interface of the physical and biological sciences.
The Genomics Facility is designated as an Ontario Genomics Platform Affiliate, OGPA, service facility and operates as a core facility offering molecular biology technology support services and training in DNA sequencing, genotyping, and gene expression analyses to the University of Guelph as well as other academic, government and private sector institutions.
Contact Us
Room 1401, Summerlee Science Complex
University of Guelph
488 Gordon St. Guelph, ON N1G 2W1
519-824-4120 ext: 58357
genomics@uoguelph.ca
Facility Hours:
Monday to Friday
9:00 am - 4:30 pm