Sanger Sequencing Fees

Sample submission considerations.

The facility provides a number of standard sequencing primers for common plasmid vectors.
Custom primers should be provided at a concentration of 5 uM (=5 pmol/uL).

Request Forms:
Individual Sample Submissions
Sanger Sequencing Request - Use this form to submit DNA templates (ie. PCR amplicons, Plasmids, etc.) for sequencing
DIY Sequencing Request - Use this for to submit completed flourescent Sanger products (ie. BigDye reactions) for sequencing

For full and half-plate submissions please contact the facility

Equipment Used: Applied Biosystems 3730 DNA analyzer

SERVICE	INTERNAL	ACADEMIA	COMMERCIAL
Single reaction	$9	$9	$16
96 Reactions (Full Plate)	$500	$600	$750
DIY Cycle Sequencing - single reaction	$5	$6	$8
DIY Cycle Sequencing - up to 48 reactions	$75	$100	$150
DIY Cycle Sequencing  - 96-well plate	$150	$200	$300
Fragment Analysis - up to 48 reactions	$50	$75	$125
Fragment Analysis - 96-well plate	$100	$150	$250

Note: 
DIY Cycle Sequencing is performed by clients with using a Sanger Sequencing Mix (ie. BigDye Terminator V3.1 Mix).
Sanger mix can be purchased separately from the facility using the AAC Consumable Order Form.

Gene Expression Services

Request Forms:
Digital Droplet PCR Request
Real-Time PCR Registration
Real-Time PCR Request

Equipment Used:
BioRad Qx200 Droplet Digital PCR (ddPCR) System
QuantStudio 7 Pro Real-Time PCR System

 

Digital Droplet PCR

Droplet Digital PCR technology is a digital PCR method utilizing a water-oil emulsion droplet system. Droplets are formed in a water-oil emulsion to form the partitions that separate the template DNA molecules.

 

SERVICE	INTERNAL
One column (8 reactions)	$45

 

RT-PCR: QuantStudio 7 Pro

This system makes simple and easy to get high-quality real-time PCR results. This automated real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.

 

SERVICE	INTERNAL
Full service	$2.50 per reaction (clients provide RNA samples and primers)
Self-service	$50 per year for registration and $5 per plate run
High-resolution melting curve	$3 per reaction (clients provide RNA samples and primers)

DNA/RNA Quantification

 

Qubit

Fluorometric concentration assays are generally more accurate than spectrophotometric/UV-Vis absorbance (ie. Nandrop) measurements because they are less affected by common contaminants (ie. Proteins, RNA, or DNA). 

This method is recommended NGS library assessments and for any application that requires higher accuracy in concentration estimates than can be acheived with Nanodrop.

 

Request Form:
Qubit Request

Equipment Used: Qubit Flex Fluorometer, Qubit® 2.0 Fluorometer

SERVICE	INTERNAL
Qubit analysis	$1.50 per sample, plus $3 for standards

 

Nanodrop

The NanoDrop^® ND-8000 can measure the concentration and quality of RNA, DNA, protein or labelled probes for microarray analysis. It is designed for the use of 1 ul samples. Cuvettes are not required. NanoDrop^® ND-8000 can measure 8 samples simultaneously. 

Subscription Form: https://www.uoguelph.ca/aac/facilities/genomics/documents/aac-geldoc-and-or-nanodrop-subscription

 

SERVICE	INTERNAL
Nanodrop	$50 per year

Agilent TapeStation 4150

The TapeStation system allows for automated electrophoresis of most DNA and RNA samples. Typical applications include quality control (QC) of NGS libraries and RNA extractions (ie. RIN).  This method excels in the determination of fragment length distribution of nucleic acid samples, but is not typically relied on for accurate quantification.  The TapeStation can be used in place of the BioAnalyzer for most applications, but cannot currently be used for small/micro RNA analysis.

Service includes Standard and High Sensitivity assays for RNA, DNA >1kb, and DNA >5kb, as well as a Standard gDNA assay.

Request Form: AAC TapeStation Request Form

Equipment Used: Agilent TapeStation 4150

Assay	Set-up	Per Sample
RNA	$10.50	$5.50
HS RNA	$12.00	$6.25
D1000	$8.00	$5.50
HS D1000	$11.00	$7.75
D5000	$12.75	$5.25
HS D5000	$17.25	$7.75
gDNA	$8.75	$8.75

 

Set-up fee is a flat fee charged per submission in addition to the per sample charge.

Set-up fee covers DNA/RNA ladders, technician time, and instrument overhead. Contact facility for more information about ladders and when to use them.