Nucleic Acid Fluorometry (Qubit)

Fluorometric concentration assays are generally more accurate than spectrophotometric/UV-Vis absorbance (ie. Nandrop) measurements because they are less affected by common contaminants (ie. Proteins, RNA, or DNA).

This method is recommended NGS library assessments and for any application that requires higher accuracy in concentration estimates than can be acheived with Nanodrop.

 

Request Form:
Qubit Request

 

Equipment Used: Qubit Flex Fluorometer, Qubit® 2.0 Fluorometer

Facility: Genomics

PromethION Services

Plasmid & PCR Amplicon Sequencing

The PromethION 24 is a high-throughput nanopore sequencer capable of natively sequencing both RNA and DNA with rapid turn-around times. The PromethION can load up to 24 flow cells, each capable of multiplexing 96 samples or more. It is also possible to re-use flow-cells, decreasing cost and waste. The PromethION 24 is suitable for long-read applications, including genome assembly, native methylation seq., direct RNA transcript sequencing (including alternate splicing), and metagenomics.

We have phased out Sanger Sequencing in favor of long-read sequencing on the Oxford Nanopore PromethION 24. The facility provides sequencing of DNA fragments up to 25 kilobases in length or more, including whole plasmids and PCR amplicons. Service starts on September 2.

  • Sequenced at the University of Guelph: no tax or shipping fees for internal clients
  • Plasmid analysis run on Tuesdays and Thursdays with next-day turnaround
  • Pricing includes bioinformatic report, plasmid annotation, insert variant calling, and multiplasmid/contamination detection.
  • See our detailed sample report
SERVICE INTERNAL ACADEMIA COMMERCIAL
Whole Plasmid $19.50 $19.50 $25
PCR Amplicon $19.50 $19.50 $25
Batch of 96 Plasmid/Amplicon $1,872 $1,872 $2,400

Sequencing Request Form - Use this form to submit DNA templates (e.g. PCR amplicons, Plasmids) for sequencing. Recommended at least 100 ng DNA. Recommended concentration 10 ng/µL in 10 µL in molecular grade water or TE buffer. Concentration should be estimated by fluorometric methods such as Qubit/PicoGreen.

  1. Fill out all three tabs of the request form (first two tabs are mandatory)
  2. Signed request form emailed to genomics@uoguelph.ca
  3. Drop the samples off at room SSC 1401 with facility staff or in the Sample Drop-Off Freezer

Other PromethION Services:

The PromethION is suitable for a range of other services, including native Methylation sequencing (or other modified bases), direct RNA sequencing, 16S profiling (or 18S/ITS/other amplicon metabarcoding), transcriptomics, whole genome sequencing, metagenomics, genome skimming, variant calling, and much more.

SERVICE INTERNAL ACADEMIA COMMERCIAL
Whole Flow Cell $1200 $1200 $1500
Other applications Contact us for quote

Equipment Used: Oxford Nanopore PromethION 24

Facility: Genomics

Protein MW Determination

MW determination for Proteins with desalting column. Samples should be in the range of 0.01-5 mg/ml in volatile buffers with minimal salt and no detergents.

Submission form for Protein MW

Equipment Used: Waters Acquity Synapt G2Si HDMS

Facility: Mass Spectrometry

Quantitative Proteomics

 

Identifying and quantitating proteins from a biological samples using protein digestion, peptide separation, protein identification, peptide intensities and statistical analysis.

Please refer to the Quantitative Proteomics page

Submission Form for Quantitative Proteomics

 

Equipment Used: Thermo Easy nLC Orbitrap Exploris 240

Facility: Mass Spectrometry

Sample Preparation

  • High Pressure Freezing
  • Plunge Freezing
  • Freeze Substitution
  • Chemical Fixation
  • Embedding
  • Sectioning
  • Staining
  • Immuno-Labeling 
  • Carbon Coating
  • Plasma Cleaning

Facility: Molecular and Cellular Imaging

Small Molecule - GC-MS Analysis

Analysis of volatiles and semi-volatiles by gas chromatography.

Sample preparation guidelines.

Sample Submission form.

Equipment Used: Agilent GC-MS

Facility: Mass Spectrometry

Small Molecule - Liquid Chromatography Mass Spectrometry

LC-MS can be used to profile and identify small molecules from a mixture. Idealy in water, ACN, MEOH, volatile buffers. Do not use or limit detergents, DMSO,DMF, high salt, non-volatile buffers or stabilizers.

Submit a minimum of 10 µl 0.1-1 mg/ml (approximately)

Submission form for high resolution LC-MS

Submission form for small molecule MW high res chemical formula confirmation

Facility: Mass Spectrometry

Small Molecule - Metabolomics (consult facility)

MSF Presentations on Metabolomics:

Metabolomics Workflows and Considerations

Metabolomics Worflow and Data Integrity

 

Refer to Metabolomics (2015) 11:851–860 for experimental design.

Software Used: Agilent Mass Profiler Professional

Submission form for metabolomics

Facility: Mass Spectrometry

Small Molecule Quantitation

Quantitation of small molecules by LC-MS requiring a pure standard(s), method development and calibration curve(s). This can also include internal standards. Standards must be provided by the cllient.

Quantitation assays are custom projects that require consultation contact dbrewer@uoguelph.ca

Submission Form for Small Molecule Quantitation

Equipment Used: Bruker EVOQ LC-TQ

Facility: Mass Spectrometry