Targeted Protein Q MS Methods
These are methods for quantitating a limited number of known proteins with high precision and accuracy in a total protein extract. This method can be particularly useful for low abundance targets.A certain amount of method development/previous data or sample preparation is required regardless of which of these methods are used.
Schematic of PRM Targeted Analysis
Discovery-based Quantitative MS methods
Quantitative Proteomics to Label or not to Label
Targeted Quantitative Proteomics MS Methods
MSF LFQ Service: Requirements and Deliverables
Further Reading:
https://proteomicsresource.washington.edu/protocols05/PRM.php