DNA Sequencing - Primers

Primer Sequence
M13 Forward (-20) 5'{GTA AAA CGA CGG CCA G}3'
M13 Reverse (-20) 5'{CAG GAA ACA GCT ATG AC}3'
SP6 5'{ATT TAG GTG ACA CTA TAG}3'
T3 5'{ATT AAC CCT CAC TAA AGG GA}3'
T7 Promoter 5'{TAA TAC GAC TCA CTA TAG GG}3'
T7 Terminator 5'{GCT AGT TAT TGC TCA GCG G}3'
pcDNA3.1/BGH Reverse 5'{TAG AAG GCA CAG TCG AGG}3'
5'-pGEX 5'{GGG CTG GCA AGC CAC GTT TGG TG}3'
3'-pGEX 5'{CCG GGA GCT GCA TGT GTC AGA GG}3'
TOPO Forward 5'{GTA AAA CGA CGG CCA G}3'
TOPO Reverse 5'{CAG GAA ACA GCT ATG AC}3'
T5 Promoter 5'{GTG AGC GGA TAA CAA TTT CAC AC}3'
pBAD Forward 5'{ATG CCA TAG CAT TTT TAT CC}3'
pBAD Reverse 5'{GAT TTA ATC TGT ATC AGG}3'

Concentration Chart

DNA Concentration (ng/ul) Provide (ng)
dsDNA 1-5 kb 50 25-125
dsDNA 5-10 kb 100 125-250
dsDNA 10-40 kb 500 500
dsDNA > 40 kb 1000 2000
PCR 100-500 bp 1-10 10-40
PCR 500-1000 bp 20-50 40-90
PCR 1000-5000 bp 50-100 90-500