AHL LabNote 55-Handling submissions requesting Equine neurological disease testing
Many clients are not familiar with testing for equine neurological disease and may ask for testing that will not provide the answers that they are looking for leading to difficulty in interpretation.
Rabies: may be on the list of rule outs. This may affect handling of fluids and tissues depending on what is submitted. If rabies is suspected (Check box or discussion with the client) please make sure that they are following up with Public Health (human involvement) or OMAFRA (animal involvement only).
Test |
Code |
Sample |
Note |
Zoonotic |
Ehv-1 Herpes (See Labnote 20) Equid herpesvirus 1-PCR (Test of Choice) |
ehv12 |
EDTA blood and/or nasal swab( VTM or swab in blood tube) preferably both EDTA blood and swab) |
This test is NOT a PCR for EHV-2 (send out to Idexx) |
N |
West Nile Virus-PCR |
wnvpn |
Brain in tripure for PCR |
If dead |
Y |
West Nile Virus IgM Elisa |
xwnveq |
Serum |
If live sent to USDA longer TAT |
Y |
Eastern Equine Encephalitis (EEEV)-PCR |
eeepn |
Brain in tripure for PCR |
If dead |
Y |
Eastern Equine Encephalitis (EEE) IgM Elisa |
xeeevme |
Serum |
live horse Sent to USDA longer TAT |
Y |
Sometimes clients ask for Virus Neutralization testing (VN) (EHV ¼, WNV, EEE, WEE. This is fine if they are looking for titres and vaccination status (Please note there are no validated guidelines for titre testing in horses) but unsuitable for acute onset testing. The only way to use titres for diagnosis is acute and convalescent titres 3-4 weeks apart submitted together for VN (a four fold rise in titre denotes infection).
Equine encephalitis virus, Eastern-PRNT (EEEV VN) |
xeeevvn |
serum |
USDA longer TAT |
Y |
Equine encephalitis virus, Western - PRNT |
xweevvn |
serum |
USDA longer TAT |
Y |
WNV VN |
xwnvvn |
serum |
USDA longer TAT |
Y |
EPM – Equine Protozoal Myelitis may also be on the list and practitioners may have their own preferred method of testing. Our standard test is:
EPM, S. neurona SAG2,4/3 titer, serum - ELISA |
xepme |
serum |
Sent to EDS longer TAT this is not a definitive test and only denotes exposure |
N |
Jim Fairles