AHL new tests and test panels developed in 2017
|
TEST NAME METHOD |
CODE |
SPECIES |
|
Avian adenovirus - PCR |
fadvrrt |
Avian |
|
Avian metapneumovirus type C (AMPV type C) - PCR |
ampvpcr |
Avian |
|
Bacillus anthracis (anthrax) - real-time PCR |
anthpcr |
Bovine |
|
Bacterial culture, aerobic and anaerobic, food animal |
ancultf |
Av, Bov, Cap, Ov, Porc, Other |
|
Bacterial culture, honey bee, American foul brood (P. larvae) |
cultbeo |
Bees |
|
Antimicrobial susceptibility, honey bee, Paenibacillus larvae |
afbo |
Bees |
|
Bacterial culture, fecal, equine |
cultnf1 |
Equine |
|
Bacterial culture, fecal, Campylobacter, add-on test |
campadd |
Av, Bov, Cap, Eq, Other, Ov, Porc |
|
Bovine abortion panel - PCR (BoHV-1/IBR, Leptospira, Neospora) |
boabopc |
Bovine |
|
Bovine enteric panel - BCoV/RotaV A/B PCR; sucrose wet mount, bacterial culture |
bentpnl |
Bovine |
|
Bovine respiratory virus panel - PCR (BoHV-1/IBR, BPIV-3, BRSV) |
brvp3 |
Bovine |
|
Bromethalin (desmethylbromethalin) - LC-MS/MS |
dmb |
Av, Bov, Can, Cap, Eq, Fel, Other, Ov, Porc |
|
Haemoplasma, non-feline, non-canine - PCR Mycoplasma haemolamae, M. ovis, M. suis, M. wenyonii |
hapcr2 |
Bovine, Other, Porcine |
|
Leptospira spp. - PCR |
leptpcr |
Bov, Can, Cap, Eq, Fel, Other, Ov, Porc |
|
Mycoplasma hyopneumoniae - gene sequencing typing |
p146seq |
Other, Porcine |
|
Myxobolus cerebralis (whirling disease pathogen) - PCR |
wdpcr |
Fish |
|
Rotavirus A&B/coronavirus, bovine/equine - PCR |
rocopcr |
Bovine, Equine |
|
Rotavirus, group A, B, C – PCR |
rotapcr |
Canine, Other, Porcine |
|
Scrapie resistance PrP genotyping, codons 136, 137, 154, 171, 176 - sequencing |
prp5 |
Ovine |
Selected zoonotic pathogens and diseases from Ontario identified at the AHL, 2017
Beverly McEwen, Durda Slavic, Davor Ojkic, Hugh Cai, Kristiina Ruotsalo, Josepha DeLay, Margaret Stalker, Murray Hazlett, Andrew Brooks, Janet Shapiro, Maria Spinato, Marina Brash, Emily Martin
Many new, emerging, and re-emerging diseases of people are caused by pathogens originating from animals, or are shared between people and animals. The AHL plays an important role in public health by identifying zoonotic pathogens in > 1,000 cases annually (Tables 1 and 2). The number and percentage of cases identified as positive for leptospirosis increased in 2017 in all species tested. These are numerator data reliant upon submission biases to the diagnostic laboratory and cannot be regarded as population prevalence estimates. Monitoring programs are not included. AHL
Table 1. Number of cases with selected zoonotic pathogens isolated and/or identified at the AHL, 2017.
|
Agent |
Bovine |
Swine |
Equine |
Ovine |
Caprine |
Chicken |
Turkey |
Canine |
Feline |
Other |
2017 |
2016 |
2015 |
2014 |
2013 |
2012 |
|
Ascarids (T. canis, T. cati, T. leonina, Baylisascaris sp.) |
0 |
12 |
10 |
0 |
0 |
23 |
2 |
16 |
5 |
9 |
77 |
41 |
17 |
40 |
36 |
35 |
|
Blastomyces dermatitidis |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
13 |
1 |
2 |
16 |
6 |
21 |
22 |
17 |
10 |
|
Bordetella bronchiseptica |
3 |
24 |
3 |
0 |
0 |
0 |
0 |
4 |
4 |
12 |
50 |
32 |
37 |
28 |
24 |
33 |
|
Borrelia burgdorferi (Lyme disease), serology |
0 |
0 |
20 |
0 |
0 |
0 |
0 |
9 |
0 |
0 |
29 |
22 |
8 |
12 |
11 |
3 |
|
Brucella sp. (non-abortus) |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
3 |
0 |
1 |
0 |
0 |
|
Campylobacter coli/ jejuni/ fetus subsp. fetus |
3 |
0 |
0 |
5 |
5 |
20 |
3 |
3 |
0 |
5 |
44 |
47 |
16 |
17 |
6 |
17 |
|
Chlamydia sp. |
1 |
0 |
0 |
5 |
11 |
0 |
0 |
0 |
0 |
1 |
18 |
13 |
24 |
15 |
25 |
33 |
|
Clostridium difficile |
1 |
8 |
4 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
14 |
5 |
10 |
11 |
11 |
19 |
|
Coxiella burnetii (Q fever) |
9 |
0 |
0 |
39 |
54 |
0 |
0 |
0 |
0 |
0 |
102 |
37 |
44 |
55 |
28 |
36 |
|
Cryptococcus sp. |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
1 |
3 |
2 |
1 |
|
Cryptosporidium sp. |
172 |
0 |
1 |
1 |
5 |
0 |
0 |
0 |
0 |
1 |
180 |
243 |
247 |
186 |
206 |
141 |
|
Eastern equine encephalitis virus |
0 |
0 |
2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
0 |
6 |
25 |
1 |
0 |
|
Giardia sp. |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
21 |
0 |
0 |
26 |
26 |
30 |
50 |
48 |
26 |
|
Listeria monocytogenes |
19 |
0 |
0 |
4 |
8 |
0 |
0 |
0 |
0 |
0 |
31 |
24 |
12 |
23 |
15 |
18 |
|
Methicillin-resistant Staphylococcus aureus (MRSA) |
2 |
0 |
9 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
11 |
12 |
28 |
17 |
8 |
24 |
|
Methicillin-resistant S. pseudintermedius (MRSP) |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
72 |
4 |
0 |
76 |
62 |
88 |
45 |
141 |
114 |
|
Rabies virus |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
0 |
0 |
0 |
0 |
|
Salmonella enterica |
64 |
67 |
8 |
8 |
3 |
27 |
35 |
24 |
2 |
28 |
266 |
290 |
332 |
221 |
308 |
281 |
|
Streptococcus suis |
10 |
102 |
2 |
2 |
1 |
1 |
1 |
0 |
0 |
1 |
120 |
181 |
167 |
105 |
126 |
144 |
|
Streptococcus equisimilis |
4 |
28 |
14 |
1 |
0 |
0 |
0 |
8 |
1 |
3 |
59 |
57 |
48 |
4 |
34 |
45 |
|
Streptococcus zooepidemicus |
0 |
2 |
121 |
2 |
0 |
0 |
0 |
2 |
2 |
4 |
133 |
154 |
138 |
93 |
112 |
4 |
|
Toxoplasma sp. |
0 |
0 |
0 |
6 |
4 |
0 |
0 |
0 |
2 |
0 |
12 |
7 |
11 |
18 |
11 |
8 |
|
Verotoxigenic E.coli (VTEC) |
5 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
6 |
12 |
8 |
7 |
18 |
|
|
West Nile virus |
0 |
0 |
15 |
0 |
0 |
0 |
0 |
0 |
0 |
43 |
58 |
18 |
19 |
6 |
44 |
36 |
|
Yersinia enterocolitica |
2 |
6 |
0 |
0 |
0 |
0 |
5 |
0 |
0 |
0 |
13 |
4 |
2 |
6 |
4 |
2 |
|
Total |
300 |
249 |
210 |
74 |
92 |
71 |
46 |
172 |
21 |
110 |
1,345 |
1,299 |
1,314 |
1,010 |
1,236 |
1,030 |
Table 2. Leptospira spp. seropositive, IHC-positive, or PCR-positive cases identified at the AHL, 2017.
|
Leptospira spp. serovar |
Bovine |
Swine |
Equine |
Canine |
Other |
|
L. autumnalis |
27 |
7 |
18 |
86 |
2 |
|
L. bratislava |
72 |
7 |
20 |
74 |
3 |
|
L .canicola |
39 |
7 |
8 |
50 |
0 |
|
L. grippotyphosa |
16 |
6 |
4 |
71 |
1 |
|
L. hardjo |
50 |
7 |
3 |
31 |
2 |
|
L. icterohaemorrhagiae |
55 |
6 |
15 |
82 |
1 |
|
L. pomona |
64 |
7 |
11 |
71 |
5 |
|
IHC, or urine PCR-positive |
1 |
1 |
0 |
10 |
0 |
|
Positive/tested cases |
91/178 |
8/27 |
26/35 |
153/230 |
5 |
|
% positive, 2017/2016 |
51/27% |
30/9% |
74/52% |
67/38% |
|
AHL Newsletter, March, 2018 - Volume 22, Number 1
Editor: Grant Maxie, DVM, PhD, Diplomate ACVP
Editorial Assistants: Helen Oliver, April Nejedly
The AHL Newsletter is published quarterly (March, June, September, December) by the Animal Health Laboratory, Laboratory Services Division, University of Guelph.
Its mission is to inform AHL clients and partners about AHL current activities, and laboratory-based animal disease events and disease trends. All material is copyright 2018. Ideas and opinions expressed herein do not necessarily reflect the opinions of the University or the Editor.
Articles may be reprinted with the permission of the editor and with appropriate credit given to the AHL Newsletter.
Mailing address & contact information:
Animal Health Laboratory
Laboratory Services Division, University of Guelph
Box 3612, Guelph, Ontario, Canada N1H 6R8
Phone: (519) 824-4120 ext. 54538; fax: (519) 821-8072
To receive an electronic copy of this Newsletter, please send your email address to us at holiver@uoguelph.ca
ISSN 1481-7179
Canada Post Publications number - 40064673
Contributors to this issue
- from the Animal Health Laboratory:
Melanie Barham, DVM, PMP
Marina Brash, DVM, DVSc, Diplomate ACVP
Andrew Brooks, DVM, PhD, Diplomate ACVP
Emily Brouwer, HBSc, DVM, Diplomate ACVP
Hugh Cai, DVM, MSc, DVSc
Michael Deane, BA
Josepha DeLay, DVM, DVSc, Diplomate ACVP
Jim Fairles, DVM, MBA
Murray Hazlett, DVM, DVSc, Diplomate ACVP
Emily Martin, DVM, MSc, Diplomate ACPV
Beverly McEwen, DVM, PhD, Diplomate ACVP
Davor Ojkic, DVM, PhD
Felipe Reggeti, DVM, PhD, Diplomate ACVP
Kristiina Ruotsalo, DVM, DVSc, Diplomate ACVP
Janet Shapiro, DVM, DipEqSurg, DipPath
Durda Slavic, DVM, PhD
Maria Spinato, DVM, DVSc, Diplomate ACVP
Margaret Stalker, DVM, PhD, Diplomate ACVP
Qiumei You, DVM, MSc
Other contributors:
Leonardo Susta, DVM PhD, DACVP, Pathobiology;, OVC.
Al Dam, BSc; Andrew Vince, DVM, DVSc, Diplomate ACVP; OMAFRA, Guelph, ON .
Our continued thanks to all of the non-author AHL clerical, technical, and professional staff who contribute to the generation of results reported in the AHL Newsletter.